Table 2.

Primers Used to Generate the Human GHR Variants, c.964dupG and c.920_921ins14

Primer Name	Primer Sequence
Primers used to generate the hGHR c.964dupG variant
hGHR c.964dupG forward	5′ GGAAGGAAAATTAGAGGAGGGTGAACACAATCTTAGCCATT 3′
hGHR c.964dupG reverse	5′ AATGGCTAAGATTGTGTTCACCCTCCTCTAATTTTCCTTCC 3′
Primers used to generate the hGHR c.920_921ins14 variant
hGHR c.920_921ins14 (step 1) forward	5′ CCCCAGTTCCAGTTCCAAAGATTAATCTCAAGGAATCGATCCA 3′
hGHR c.920_921ins14 (step 1) reverse	5′ TGGATCGATTCCTTGAGATTAATCTTTGGAACTGGAACTGGGG 3′
hGHR c.920_921ins14 (step 2) forward	5′ CAGTTCCAGTTCCAAAGATTAATCTCAAAGATAGGAATCGATCCAG 3′
hGHR c.920_921ins14 (step 2) reverse	5′ CTGGATCGATTCCTATCTTTGAGATTAATCTTTGGAACTGGAACTG 3′
hGHR c.920_921ins14 (step 3) forward	5′CCAGTTCCAAAGATTAATCTCAAAGATTACAAGGAATCGATCCGATC 3′
hGHR c.920_921ins14 (step 3) reverse	5′ GATCTGGATCGATTCCTTGTAATCTTTGAGATTAATCTTTGGAACTGG 3′

Nucleotides inserted are underlined.