FIG 1.

EBOV growth kinetics within taxonomically diverse cell lines. (A) Transcription of EBOV in EhKiT, HEK293T and PK15A cells was quantified at 6 hpi and 24 hpi by mapping RNA-seq reads against the EBOV genome sequence (represented as blue arrows at the top). The coverage was normalized per million reads mapped to the host genomes. The normalized coverage was averaged across the three biological replicates at each time point. Note the different scales on the y axes. (B) Log₁₀ number of copies of EBOV NP mRNA determined by TaqMan PCR across the three cell lines at 6 and 24 hpi. The mean ± standard error from three independent biological replicates is presented, with each replicate being performed as a duplicate PCR. (C) Western blot of EBOV NP protein in the three cell lines at 0, 6, and 24 hpi. Western blotting was performed from the three independent biological replicates, with a representative image being shown here. (D) The replication of EBOV was quantified as the number of TCID₅₀ per milliliter at 24, 48, and 72 hpi for each cell line. The mean ± standard error from three independent biological replicates is presented.