FIG 11.

Response of HEK293T cells to purified EBOV. (A) Relative expression of FOS, JUN, ATF3, and CYR61 mRNA following infection by purified EBOV for 24 and 48 h. The mean ± standard error from three independent biological replicates is presented, with each replicate being performed as a duplicate PCR. (B) The levels of expression of total and phosphorylated forms of the JUN protein were assessed by Western blotting. β-Tubulin was used as a loading control. Western blot analysis was performed in duplicate from two independent experiments, with a representative image being shown here. *, P < 0.05; ***, P < 0.001.