FIG 5.

Comparison of interferon response across cell lines. (A) Heatmap representation of individual genes contributing to the enriched GO term cytokine-mediated signaling pathway (GO:0019221). Each tile represents the mean log₂ fold change in regulation from three independent biological replicates. Gray tiles represent genes that had no human ortholog or had an infinity fold change (i.e., no reads at 0 hpi). (B and C) Relative IFNB1 (B) and IFIT1 (C) mRNA expression (as the log₂ fold change) for HEK293T, PK15A, and EhKiT cells following transfection with poly(I·C) for 2, 4, and 6 h. The mean ± standard error from three biological replicates is presented, with each replicate being performed as a duplicate PCR. (D and E) Relative IFNB1 (D) and IFIT1 (E) mRNA expression (as the log₂ fold change) for HEK293T, PK15A, and EhKiT cells following treatment with type I universal interferon (IFN). The mean ± standard error from three biological replicates is presented, with each replicate being performed as a duplicate PCR.