AP1 activity is induced by EBOV in HEK293T cells. The transactivation activity of AP1 was assessed following EBOV infection for 24, 48, and 72 hpi using a dual-luciferase reporter assay in HEK293T cells. Assays with PMA-induced controls (50 ng/ml) were performed at each time point, and the results are represented on the right y axis. The mean fold increase (relative to that for the uninfected control) ± standard error from three independent biological replicates is presented, with each replicate being performed as a duplicate luciferase assay. *, P < 0.05; ***, P < 0.001.