FIG 4.

Higher proportion of VP1-31G viruses from fatal cases than HFMD cases. (a) The Shannon entropies of quasispecies isolated from patients with fatal disease or HFMD were compared based on the frequency of distribution of all haplotypes. The 1998-1 virus was isolated from a throat swab of an autopsy case. The center values represent the Shannon entropies among the selected fatal and HFMD cases. The proportions of VP1-31G (b) and 3A-73S (c) among virus isolates from patients with fatal disease or HFMD were determined based on the SNV calling results from deep-sequencing reads. The values represented by the horizontal lines are mean proportions among the selected fatal and HFMD cases. The Mann-Whitney U test was used to calculate P values. *, P < 0.05. (d) The πN values for EV-A71 from fatal cases and HFMD cases were calculated for each viral genome using a 50-codon sliding window and a 1-codon step.