FIG 1.

Terminally differentiated neuronal SH-SY5Y cells are morphologically distinct from undifferentiated SH-SY5Y cells and SK-N-SH cells. (A) Terminally differentiated SH-SY5Y cells appear neuronal after 2.5 weeks of differentiation. At 2 days postplating, mixed cell morphologies are visible in undifferentiated SH-SY5Y cells (B) and in the parent cell line SK-N-SH (C). Black arrows indicate epithelial-like cells, and red arrowheads indicate neuronal-like cells. (D) Traced outlines of representative cells, approximately 1.5 times their actual size. Scale bars represent 100 μm.