FIG 5.

Neuronal SH-SY5Y cells reveal distinct phenotypes of cell-to-cell spread for different strains of HSV-1. Following a low-MOI infection with sample collection at 24, 48, and 72 hpi, neuronal SH-SY5Y cells (A) but not undifferentiated SH-SY5Y cells or Vero cells (B) distinguish between two phenotypically distinct viral strains. For these multistep growth curves, all three cell types were infected with HSV-1 McKrae (green) or HSV-1 KOS (orange) at an MOI of 0.01 in the presence of 0.1% pooled human serum. Data include three biological replicates, and error bars represent the SEM. Multiple t tests were performed for neuronal SH-SY5Y cells, with alpha of 5.0%. *, P = 0.04.