FIG 6.

Differentiated neuronal cells have a unique pattern of cGAS expression compared to MRC5 cells before and during HSV-1 infection. Differentiated neuronal cells express the antiviral protein cGAS in the cytoplasm and in neurites both before and after HSV-1 infection, while MRC5 cells express cGAS and IFI16 in the nucleus following HSV-1 infection. (A) Terminally differentiated SH-SY5Y neuronal cells were infected at an MOI of 10 with HSV-1 McKrae and then fixed and processed for cGAS expression at 3 hpi or 6 hpi. Scale bars represent 20 μm. The zoomed-in images were acquired with a 168× objective, and scale bars represent 10 μm. (B) MRC5 fetal lung fibroblasts were infected at an MOI of 10 with HSV-1 McKrae and then fixed and processed for immunofluorescence at 3 hpi. Scale bars represent 20 μm. The zoomed-in images were acquired with a 156× objective, and scale bars represent 10 μm. Images were taken with an Olympus FV10i confocal microscope with a 60× objective.