FIG 1.
Development of DENV1 VLPs. (A) Schematic illustration of the DENV genome and DENV1 VLP expression vectors. C, capsid; ss, signal sequence; prM, precursor membrane protein; E, envelope. (B) Western blot analysis of DENV1 VLP-containing culture supernatant. 293F cells were transfected with the control vector or an expression plasmid encoding wild-type DENV1 prM-E (prM-E) or prM-E with the F108A mutation (prM-EF108A) and then cultured for 4 days. Secreted DENV1 VLPs were detected using a mouse anti-DENV1-4 E monoclonal antibody. The intensity of each band was measured, and the relative intensity was calculated by setting the intensity of prM-E to 1.0. An image representative of at least 3 independent experiments is shown.