FIG 7.

CD19 deficiency does not impair recruitment of IgD⁺ B cells into the CNS. Brains from infected WT and CD19^−/− mice were analyzed for accumulation of total CD45^hi cells, as well as undifferentiated IgD⁺ B220⁺ B cells, by flow cytometry. (A) Representative density plots showing CD45 staining and the side scatter (SSC) pattern and gating strategy for B220⁺ cells within infiltrating CD45^hi cells. (B and C) Percentages of B220⁺ cells within the CD45^hi infiltrating population (B) and percentages of IgD⁺ cells among CD45^hi B220⁺ B cells (C) over time. The data represent the means and SEM of individual mice from 2 separate experiments, each comprising 2 to 4 individual mice per time point and group. Statistically significant differences between WT and CD19^−/− mice are denoted by asterisks: **, P < 0.01.