FIG 4.

Interactions between the Nipah virus and the PRP19 complex. (A) Network representation of TAP-MS-identified interactions between PRP19 complex members (gray nodes) and Nipah virus proteins (red nodes). Solid black lines indicate interactions found in the TAP-MS experiments. Gray dotted lines represent PPIs identified in the String and intACT databases. (B) Average log(emPAI/median) value of the experimentally found interactions. Red dots highlight the PRP19 complex-associated proteins. The dotted line indicates the average value for all samples. (C) Western blot identification of PRP19 complex members. Wild-type and mutant (G121E) V and W lysates, together with EYFP, were purified by TAP and the final eluates immunoblotted for CDC5L, PRP19, PLRG1, BCAS2, and the Flag tag. (D) Cellular localization of the V, W, and P proteins (red; immune labeled) with YFP-PLRG1 (green). DAPI staining is shown as light gray. Colocalization of red and green channels is highlighted in yellow. Arrowheads indicate perinuclear regions where green and red colocalization was found.