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. 2017 Nov 14;91(23):e01461-17. doi: 10.1128/JVI.01461-17

TABLE 1.

Proteins identified by the TAP-MS

Bait UniProt accession no. Gene name Protein name No. of replicatesa Found ratiob No. of replicates inc:
 Avg log(emPAI/med)d Referencee
Nt Ctc
W O00505 KPNA3 Importin-alpha 3 3 0.75 1 2 1.59 22, 37
P O75934 SPF27 Pre-mRNA-splicing factor spf27 4 1.00 2 2 1.43
P Q9UMS4 PRP19 Pre-mRNA-processing factor 19 4 1.00 2 2 1.37
M Q92688 ANP32B Acidic leucine-rich nuclear phosphoprotein 32 2 0.50 1 1 1.28 38
W O00629 KPNA4 Importin subunit alpha-4 4 1.00 2 2 1.26 22, 37
W B2R7W3 BCAS2 Pre-mRNA-splicing factor spf27 4 1.00 2 2 1.23
W Q9UMS4 PRP19 Pre-mRNA-processing factor 19 4 1.00 2 2 1.19
P Q99459 CDC5L Cell division cycle 5-like protein 4 1.00 2 2 1.18
V B2R7W3 BCAS2 Pre-mRNA-splicing factor spf27 3 0.75 1 2 1.08
V P52630 STAT2 Signal transducer and activator of transcription 2 2 0.50 1 1 0.98 16, 37
V Q9UMS4 PRP19 Pre-mRNA-processing factor 19 4 1.00 2 2 0.94
P O43660 PLRG1 Pleiotropic regulator 1 4 1.00 2 2 0.89
C P40855 PEX19 Peroxisomal biogenesis factor 19a 2 0.50 2 0 0.83
F Q53H37 CALM5 Calmodulin-like protein 5 2 0.40 0 2 0.80
C P35613 BSG Basigin/cd147emmpirin 3 0.75 2 1 0.80
V O43660 PLRG1 Pleiotropic regulator 1 2 0.50 0 2 0.79
F Q9Y5M8 SRPRB Signal recognition particle receptor subunit beta 3 0.60 1 1 0.78
N O75190 DNAJB6 DnaJ subfamily b member 6 2 0.33 1 1 0.76
C Q9UHG3 PCYOX1 Prenylcysteine lyase 2 0.50 2 0 0.73
M P49458 SRP9 Signal recognition particle 9-kDa protein 2 0.50 1 1 0.67
M A0A024R2I7 RAD18 Postreplication repair protein hrad18p 4 1.00 2 2 0.67 32
W O43660 PLRG1 Pleiotropic regulator 1 3 0.75 1 2 0.66
F I2G9F8 HLA-C Major histocompatibility complex class I-c 3 0.60 2 1 0.62 39*
F P11441 UBL4A Ubiquitin-like protein 4a 4 0.80 2 1 0.59
P P51116 FXR2 Fragile x mental retardation syndrome-related protein 4 1.00 2 2 0.55
V P42771 CDKN2A P16-ink4 2 0.50 1 1 0.48
N D3DR22 HSD17B12 Hydroxysteroid (17-beta) dehydrogenase 12 2 0.33 2 0 0.48
C O00161 SNAP23 Snap-23 2 0.50 2 0 0.47
M O15355 PPM1G Protein phosphatase 1g 4 1.00 2 2 0.47
V Q99459 CDC5L Cell division cycle 5-like protein 3 0.75 1 2 0.36
G P07203 GPX1 Glutathione peroxidase 2 0.40 1 1 0.33
N M4QFU4 HLA-B Major histocompatibility complex class I antigen 2 0.33 0 2 0.33 39*
M Q9UPY3 DICER1 Hypothetical helicase k12h4.8-like protein 2 0.50 0 2 0.30
M Q7Z6Z7 HUWE1 E3 ubiquitin-protein ligase huwe1 2 0.50 0 2 0.28
M Q8WVZ9 KBTBD7 Kelch repeat and btb domain-containing protein 7 2 0.50 1 1 0.27
W Q99459 CDC5L Cell division cycle 5-like protein 3 0.75 1 2 0.26
F A0A024RBE6 NAP1L1 Nucleosome assembly protein 1-like 1, cra_b 2 0.40 0 2 0.25
M Q15633 TARBP2 RISC-loading complex subunit tarbp2 b 3 0.75 1 2 0.23
C Q9P0T7 TMEM9 Transmembrane protein 9 2 0.50 0 2 0.23
N O00217 NDUFS8 NADH dehydrogenase iron-sulfur protein 8 2 0.33 2 0 0.22
G Q15768 EFNB3 Ephrin-b3 2 0.40 2 0 0.19 32
C Q9H9H4 VPS37B Vacuolar protein sorting-associated protein 37b 4 1.00 2 2 0.19
V Q13126 MTAP Methylthioadenosine phosphorylase 3 0.75 1 2 0.18
F Q7L5D6 GET4 Cgi-20 protein 2 0.40 2 0 0.14
F A4D0U5 TES Testin 1 4 0.80 2 2 0.14
G Q9UK22 FBX2 F-box protein Fbx2 5 1.00 2 2 0.13
N Q9Y4R8 TELO2 Kiaa0683 protein 2 0.33 1 1 0.12
F Q3SY69 ALDH1L2 Aldehyde dehydrogenase 1 family, member l2 2 0.40 1 1 0.09
M Q9UL15 BAG5 Bag family molecular chaperone regulator 5 2 0.50 1 1 0.06
P O43709 WBSCR22 Uncharacterized methyltransferase wbscr22 2 0.50 1 1 0.05
G P27544 CERS1 Ceramide synthase 1 2 0.40 2 0 0.05
G O60613 SEP15 Selenoprotein 1 precursor 2 0.40 2 0 0.05
C Q9BRK5 SDF4 Calcium-binding protein 1 precursor 2 0.50 0 2 0.03
F P51648 ALDH3A2 Fatty aldehyde dehydrogenase 2 3 0.60 1 1 0.03
F Q96AY3 FKBP10 Fk506-binding protein 2 0.40 2 0 0.01
N Q8NBQ5 HSD17B11 Unnamed protein product 3 0.50 1 2 0.01
P P52630 STAT2 Signal transducer and activator of transcription 2 2 0.50 1 1 0.01 16, 37
F P02786 TFRC Transferrin receptor protein 1 3 0.60 2 1 0.00 40**
G P52799 EFNB2 Ephrin-b2 2 0.40 2 0 −0.01 8, 41
M Q6PEV8 FAM199X Fam199x 2 0.50 0 2 −0.02
M Q9Y4B6 VPRBP Vprbp 1 3 0.75 1 2 −0.04 42
G Q9Y3A6 TMED5 Transmembrane emp24 protein transport 3 0.60 0 2 −0.06
F P46459 NSF Vesicle-fusing ATPase 2 0.40 2 0 −0.09
C P51648 ALDH3A2 Fatty aldehyde dehydrogenase 2 2 0.50 1 1 −0.09
C Q9NV96 TMEM30A Cell cycle control protein 50a 2 0.50 0 2 −0.10
F Q9UNL2 SSR3 Translocon-associated protein subunit gamma 3 0.60 1 1 −0.10
W Q14765 STAT4 Signal transducer and activator of transcription 4 3 0.75 2 2 −0.10
P P56182 RRP1 Ribosomal RNA processing protein 1 2 0.50 1 1 −0.11
M Q9BTT0 ANP32E Acidic leucine-rich nuclear phosphoprotein 32 2 0.50 1 1 −0.12
C P25490 YY1 Transcriptional repressor protein yy1 2 0.50 0 2 −0.13
N Q9H078 CLPB Caseinolytic peptidase b 3 0.50 2 1 −0.14
N Q8WVC6 DCAKD Dephospho-coenzyme A kinase domain containing 3 0.50 3 0 −0.15
N O75306 NDUFS2 NADH dehydrogenase-ubiquinone Fe-S protein 2 3 0.50 1 2 −0.15
F Q7L099 RUFY3 Protein Rufy3 2 0.40 0 2 −0.16
G O15173 PGRC2 Membrane-associated progesterone receptor component 2 3 0.60 2 0 −0.16
F Q15165 PON2 Paraoxonase 2 2 0.40 2 0 −0.16
C Q9H4A5 GOLPH3L Golgi phosphoprotein 3-like 2 0.50 2 0 −0.16
M Q9GZU8 FAM192A Protein fam192a 3 0.75 2 1 −0.16
M A0A0C4DGV5Z RANB2 Zis1 4 1.00 2 2 −0.20
N P10155 TROVE2 Ro ribonucleoprotein 4 0.67 3 1 −0.21
M Q7Z4V5 HDGFRP2 Hepatoma-derived growth factor-related protein 2 2 0.50 1 1 −0.22
M Q96JK2 DCAF5 Ddb1- and Cul4-associated factor 5 2 0.50 0 2 −0.22
C Q9ULX6 AKAP8L A-kinase anchor protein 8-like 2 0.50 1 1 −0.25
M Q96EY7 PTCD3 Pentatricopeptide repeat domain-containing protein 3 4 1.00 2 2 −0.25
C O95071 UBR5 E3 ubiquitin-protein ligase Ubr5 2 0.50 0 2 −0.25
N Q8IWV7 UBR1 E3 ubiquitin-protein ligase Ubr1 4 0.67 1 3 −0.26
M O00203 AP3B1 Ap-3 complex beta3a subunit 2 0.50 1 1 −0.29 42
M O43164 PJA2 Praja ring finger 2 3 0.75 1 2 −0.30
F Q8IXB1 DNAJC10 Hypothetical protein 2 0.40 1 1 −0.32
C Q8TEB1 DCAF11 Gl014 3 0.75 2 1 −0.32
C O43933 PEX1 Peroxisome biogenesis factor 1 2 0.50 0 2 −0.36
G Q9NRX5 SERINC1 Kiaa1253 protein 2 0.40 2 0 −0.38
N Q96AC1 FERMT2 Mitogen-inducible gene mig-2 3 0.50 0 3 −0.39
C Q13557 CAMK2D Calcium/calmodulin-dependent protein kinase 2 delta 2 0.50 0 2 −0.39
M P30414 NKTR NK-tumor recognition protein 2 0.50 1 1 −0.50
N A0A024RCG7 ARMCX2 Armadillo repeat containing 6 3 0.50 2 1 −0.54
N I0B0K5 FLG Truncated profilaggrin 2 0.33 1 1 −0.56
M P07199 CENPB Cenp-b 2 0.50 0 2 −0.62
N Q5VYK3 ECM29 Proteasome-associated protein Ecm29 2 0.33 1 1 −0.67
F Q5T5U3 ARHGAP21 Rho-GTPase activating protein 10 2 0.40 1 1 −0.94
C Q92621 NUP205 Nuclear pore complex protein nup205 3 0.75 2 1 −0.97
a

Number of replicates in which the protein were found.

b

Number of replicates in which the protein was found/total number of replicates performed with the indicated bait.

c

Number of replicates in which the protein was found with the Nt or Ct TAP tag.

d

Proteins were sorted based on the average log(emPAI/med).

e

*, PPI reported with other Nipah virus protein; **, PPI reported with the equivalent Hendra virus protein.