FIG 9.

Sensitive monitoring of intracellular and secreted levels of NanoLuc-tagged NS1 following infection with DENV2-NS1-NLuc. (A) Huh-7.5 cells in 96-well plates were infected with DENV2-NS1-NLuc at the indicated MOI for 4 h, washed, and returned to culture for 48 h prior to the determination of intracellular and extracellular NanoLuc activity. (B) Semiconfluent Huh-7.5 cells in 96-well trays were treated with the indicated concentration of nanchangmycin for 1 h prior to infection with DENV2-NS1-NLuc (MOI of ∼0.05) for 4 h. Cells were then washed and returned to culture for 48 h prior to measurement of intracellular and extracellular NLuc activity. Data are means ± the (n = 4). The results are representative of similar repeat experiments.