FIG 3.

HIV DNA is inversely correlated with induced IFN-γ production in NK cells from HIC. (A to C) Linear correlation analysis of quantitated integrated HIV DNA (copies/10⁵ CD4⁺ cells) and purified NK cell IFN-γ production upon triggering with activating receptors or cytokines. (A) Anti-NKp46 and anti-NKp30. Data are representative for 17 patients. (B) Anti-NKp30 and anti-DNAM-1. Data are representative for 16 patients. (C) rhIL-12 plus rhIL-15. Data are representative for 12 patients. (D and E) Flow cytometric analysis of peripheral NK cells producing IFN-γ from two patients with widely diverging HIV DNA copy numbers (marked with open diamonds and open triangles in panels A to C), i.e., high (D) and low (E) HIV DNA copy numbers. CD3⁻ cells were gated by flow cytometric analysis and analyzed for CD56 and CD16 expression and for intracellular IFN-γ production.