FIG 9.
Purified NK cells from HIC patients control HIV integration in vitro. IL-2/PHA CD4+ T lymphocytes were infected with HIV-1Bal at an MOI of 0.01 in the presence or absence of activated NK cells from PP or HIC. After 7 days of culture, virus replication was monitored by measuring HIV RNA (copies per milliliter) in the supernatant (A), p24 levels via intracellular staining of CD3+56− T cells by flow cytometry (B), and viral reservoir accumulation by quantitative assay of total HIV DNA (copies/105 CD4+ cells) (C) and of integrated HIV DNA (D). (E) Fold decreases in virus accumulation in the presence of the given purified NK cells over that in control cultures without NK cells. (F) Relative potencies of purified NK cells from HIC versus NK cells from PP in inhibiting virus replication or DNA integration/accumulation. HIC, HIV controller patients (n = 6); PP, progressor patients (n = 6).