FIG 9.

Phosphorylation of BKRF4. (A) HEK293 cell clones that latently maintain the wild type or BGLF4 knockout (BGLF4-KO) recombinant EBV-BAC genome were transfected with the BZLF1 expression vector by electroporation and harvested on days 0 and 2. Cell lysates were treated with or without lambda phosphatase and separated by Phos-tag gel electrophoresis (Phos-tag) or conventional SDS-PAGE, followed by IB with anti-BKRF4, anti-BGLF4, and anti-tubulin antibodies. (B) HEK293T cells were transfected with the expression vectors by lipofection. After 1 day, the cells were harvested and treated as in panel A and subjected to IB with anti-BKRF4, anti-BGLF4, anti-Flag, and anti-tubulin antibodies.