FIG 1.

Antiapoptotic effects of ks-vFLIP overexpression in Jurkat cells. Viability assays were conducted with Jurkat cells treated with various concentrations of ETO (A) and TNF-α (B). The ability of ks-vFLIP to protect Jurkat cells against the effects of ETO (C) and TNF-α (D) where FADD and ATG3 have been silenced using siRNA, together with those null for IKKγ, was assessed. (E) (Left image) Immunoblot comparing ks-vFLIP levels in Jurkat vFLIP cells with those in the KSHV-transformed PEL cell line BC3. (Right and middle images) Downregulation of FADD and ATG3 in Jurkat cells using siRNA. Asterisks indicate significant differences (*, P < 0.05, **, P < 0.01, ***, P < 0.001; z test). ns, not significant.