FIG 7.

M1 inhibits yeast lethality that is triggered by a reconstituted apoptosome or constitutively active caspase-9. Yeast cells were transformed with the indicated expression plasmids, and lethality was induced by overexpressing either Apaf-1, procaspase-9 (Casp-9), and procaspase-3 (Casp-3) or Bax (A) or reverse caspase-9 (rCasp-9) and procaspase-3 (B). Suspensions containing equivalent concentrations of each transformant were serially diluted, and 5 μl of each dilution was spotted onto plates containing galactose (to induce transgene expression) or glucose (to repress transgene expression). Growth on inducing plates indicates survival and proliferation of yeast cells expressing the transgenes.