FIG 2.

Genotype (A) and plaque morphology (B) of five reassortant clones of HPAIV H5N1 and H9N2 strains from Egypt generated by coinfection of embryonated chicken eggs. (A) The eight viral genome segments are indicated by dashes, which indicate, from top to bottom, PB2, PB1, PA, HA, NP, NA, M, and NS segments. Gene segments of HPAIV H5N1 parent strain A/chicken/Egypt/AR236/2015 (AR236) are colored in green, and those of the H9N2 subtype are shown in purple. The genotypes of five reassortant clones (C5, C8, C11, C20, and C46, all of the H5N1 subtype) are shown. (B) The size of 25 randomly selected plaques per clone in infected MDCK cells was measured using ImageJ software. The plaque size of parent strain AR236 was considered 100%. *, statistically significant differences in relation to the parental virus (AR236) (P < 0.001).