FIG 5.

Oropharyngeal and cloacal shedding of different reassortant clones (C5, C8, C11, C20, and C46) and of the HPAIV H5N1 parent strain (A/chicken/Egypt/AR236/2015) in 8-week-old White Leghorn specific-pathogen-free chickens at day 2 postinfection. Six groups of 8 week-old SPF chickens were inoculated oculonasally with 10⁵ TCID₅₀ of five reassortant clones (C5, C8, C11, C20, and C46) or with the H5N1 parent strain (AR236). Viral shedding in oropharyngeal and cloacal swab specimens at 2 dpi was determined. The y axis represents the calculated TCID₅₀ milliliter⁻¹ equivalents. The x axis indicates the different reassortant clones. Within each box plot, the center line represents the median, while the top and bottom borders mark the 75th and 25th percentiles, respectively. Whiskers represent the minimum and maximum values. Extreme outliers are indicated by circles.