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. 2017 Nov 14;91(23):e01300-17. doi: 10.1128/JVI.01300-17

FIG 7.

FIG 7

(A, B) C11-infected ferret lung at 4 dpi. (A) Mild, acute, catarrhal bronchiolitis with protein-rich fluid, desquamated and degenerating epithelia (arrow), and scant neutrophils within the lumen. (B) Focal spot of influenza A virus nucleoprotein-immunoreactive cellular debris, epithelial cells, and alveolar macrophages interpreted as remnants of a necrotic bronchiolus. (C, D) AR236-infected ferret lung at 4 dpi. (C) Moderate, acute, catarrhal and suppurative (broncho-)pneumonia with intra-alveolar neutrophils (arrow), macrophages (arrowhead), and protein-rich edema. (D) Multifocal accumulation of influenza A virus nucleoprotein-immunoreactive cellular debris, alveolar macrophages, and/or type II pneumocytes. (E, F) C46-infected ferret nasal conchae at 4 dpi. (E) Mild, acute, focal degeneration of epithelial cells (arrow) within the respiratory mucosa. (F) Multiple influenza A virus nucleoprotein-immunoreactive epithelial cells within superficial and deeper layers of the respiratory mucosa. (G, H) C46-infected ferret lung at 4 dpi. (G) Moderate, subacute, coalescing, proliferative pneumonia with hyperplastic type II pneumocytes (arrow) and alveolar histiocytosis. (H) A discrete, large round cell with an influenza A virus nucleoprotein-immunoreactive nucleus and cytoplasm interpreted to be an alveolar macrophage or desquamated type II pneumocyte. (A, C, E, G) Hematoxylin and eosin stain. (B, D, F, G) Influenza A virus nucleoprotein immunohistochemistry by the avidin-biotin-peroxidase complex method with 3-amino-9-ethylcarbazol as the chromogen and hematoxylin counterstain. Bars = 20 μm.