FIG 1.

Mature MDDCs have low susceptibility to HIV-1 infection, which is blocked at the reverse transcription level. (A) p24 measured in culture supernatants after the infection of immature MDDCs (iMDDCs; blue) and mature MDDCs (mMDDCs; red) with HIV-1 BaL in 3 independent experiments. The means and standard deviations for three replicates are shown at each time point. (B and C) Luciferase activity (B) or percentage of GFP⁺ cells (C) in mature MDDCs compared with that in immature MDDCs after infection with single-cycle HIV-1 NL4.3Δenv VSV-G particles carrying luciferase (n = 16) or GFP (n = 6) reporter genes. Each symbol represents results with cells from different donors. The median and IQR values are shown. (Right) One representative example of GFP production in immature and mature MDDCs is shown. (D and E) Relative number of integrated Alu-long terminal repeat (LTR) copies (D) or U5-Gag copies (E) quantified by RT-qPCR in mature MDDCs compared with that in immature MDDCs 72 h after infection with single-cycle HIV-1 NL4.3ΔenvLuc VSV-G particles. Viral cDNAs were normalized to the albumin gene content in each sample. Experiments with cells from three different donors are shown. Bars represent the means from 2 replicates.