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. 2017 Nov 14;91(23):e01324-17. doi: 10.1128/JVI.01324-17

FIG 3.

FIG 3

Downregulation of p21 expression levels by siRNA in mature MDDCs restores viral replication. (A and B) The relative number of copies of p21 mRNA (A) and the relative p21 protein levels (B) in immature and mature MDDCs after transfection with siRNA targeting p21 (siRNA p21) or a pool of nontargeting siRNA (siRNA NT). The data are expressed in relation to the number of mRNA copies or protein levels in immature MDDCs transfected with nontargeting siRNA. Symbols represent experiments with cells from different donors (n = 8 for mRNA and n = 4 for protein). The median and IQR values are shown. An example of Western blot analysis of the different proteins studied is shown on the right. (C) Level of expression of CD86, CD80, and CD40 on the surface of MDDCs transfected with siRNA NT or siRNA p21 and cultured in the absence or presence of CD40L and IFN-γ. (D) Changes in luciferase activity in immature and mature MDDCs transfected with siRNA NT or siRNA p21 after infection with HIV-1 NL4.3ΔenvLuc VSV-G. The results are shown as the fold change relative to the luciferase activity in immature MDDCs transfected with siRNA NT. Symbols represent independent experiments (n = 8 donors). The median and IQR values are shown. One representative experiment is shown on the left (means and standard deviations from three replicates). P values of ANOVA are shown. Pairwise significant differences (P < 0.05) in post hoc analyses are identified by horizontal lines.