FIG 4.

Maturation of MDDCs decreases the size of the intracellular dNTP pools, but the pool sizes recover upon p21 knockdown. (A) Intracellular levels of dATP, dCTP, dGTP, and dTTP quantified by single-nucleotide primer extension gel analysis in immature and mature MDDCs. Independent experiments with cells from 5 donors are shown. (B) Changes in the dNTP levels in immature and mature MDDCs transfected with siRNA NT or siRNA p21. The results are shown as fold change relative to each dNTP level in immature MDDCs transfected with siRNA NT. The median and IQR values of the results obtained with cells from 4 different donors are shown (n = 4 donors). P values of ANOVA are shown. Significant differences (P < 0.05) between conditions in post hoc analyses are identified by horizontal lines.