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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Biochemistry. 2015 Jul 24;54(30):4711–4730. doi: 10.1021/acs.biochem.5b00362

Figure 12. Binding of rCvGal1 and rCvgal2 to Perkinsus parasites.

Figure 12

P. marinus (Pm) or P. chespeaky (Pc) were fixed and incubated with 0–10 μg/ml of rCvGal2 (bottom), which was pre-incubated with (0.1) or without (0) 0.1 M of lactose, for 1h at RT. The cells were stained respectively with anti-CvGal2 antibody followed by FITC-conjugated secondary antibody; and the binding of recombinant proteins was revealed by flow cytometry. Data are shown as MFI±95%CI. p<0.05; **: p<0.01; ***: p<0.001 vs. no rCvGal2 (0); Significant differences between two samples are shown in bar with p values.