Table 1.
Primers used for CvGal2 genomic DNA analysis
| Primer | Sequence | mRNA position | DNA position | Mark* | Seg. |
|---|---|---|---|---|---|
| CA1-F | CGGTTCAAGTCAAAGGCGAT | 1296–1315 | Exon 10 | amp | 5th |
| CA3-R | CCCTTCTGATGGTCGTGGAT | 289–270 | Exon 4 | amp | 1st |
| CA4-F | CATTTCAACGTTCGGAGGGG | 1493–1512 | Exon 12 | amp | 6th |
| CA4-R | AAGTTGCAGTCTCCGTCCAT | 1737–1718 | Exon 13 | amp | 5th |
| CA5-F | CTCCGTGTGGTTTTCTTCGC | 24–43 | Exon 1 | amp | 1st |
| CA5-R | ATTCGTATATTATCATAGTGCAGC | 1922–1945 | Exon 13 | amp | 6th |
| CA7-F | TTGTAACACTCGCACTTGGG | Intron1 | seq | 1st | |
| CA7-R | TTTTCCTGCCTCTCTGATCC | 112–93 | Exon 2 | seq | 1st |
| CA8-F | TTCACCCGATGCATGGTAGT | Intron 3 | amp | 2nd | |
| CA9-F | ACAACCAGACATGCATGACT | Intron 3 | seq | 2nd | |
| CA9-R | ATGTTCCTGGCCGTATTCCT | 1381–1400 | Exon 11 | amp | 4th |
| CA10-F | CTCGCCATCTATCCACGTCT | Intron 1 | seq | 1st | |
| CA10-R | ACCTTCCGTTTTTCCTGCCT | 101–118 | Exon 2-Intron 2 | seq | 1st |
| CA11-F | GGGCGGGAAAGTACCTCATC | 468–487 | Exon 4 | amp | 3rd |
| CA11-R | CTAACCACGGTCCTCTGTGC | 702–683 | Exon 6 | amp | 2nd |
| CA12-F | GGCGAGGATGCAATGA | 938–953 | Exon 8 | amp | 4th |
| CA12-R | CCGCGGATTGAAGTGAAGA | 1094–1075 | Exon 9 | amp | 3rd |
| CA14-R | GATGTTGTTCCGGTTGTCATTG | 658–637 | Exon 6 | seq | 3rd |
| CA15-R | CGGATTTCCGCGGTGTGTAA | 849–868 | Exon 7 | seq | 3rd |
| CA16-R | TTTCAGTGGGTTTACCAAGG | Intron 4 | seq | 3rd |
*
amp: for segment amplification; seq: for sequence