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. 2017 Nov 14;10:269. doi: 10.1186/s13068-017-0964-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Measured EPR spectra of (a) BsCel5A-CBM3_C405R1 and (b) BsCel5A-RtCBM11_Y151R1 both free in solution and in the presence of β-glucan. The spectra are superimposed and normalized to the total area to emphasize the difference in line width and shape. The arrows highlight the increase in the out-peak separation of the spectrum due to β-glucan binding and immobilization of the spin-labeled side chain