Table 1.

Kinetic parameters for β-glucan and CMC hydrolysis by cysteine mutants BsCel5A-CBM3 and BsCel5A-CBM11 enzymes

Enzyme	Substrate	V max (U mg−1)	K 0.5 (mg mL−1)	K cat (s−1)	K cat/K 0.5 (mL mg−1 s−1)
BsCel5A-CBM3	β-glucan	498.1 ± 20.1	2.88 ± 0.05	472.34 ± 20.1	164.0
	CMC	218.7 ± 10.4	6.97 ± 0.15	199.70 ± 10.4	28.65
BsCel5A-RtCBM11	β-glucan	985.7 ± 40.2	2.66 ± 0.04	934.72 ± 40.2	351.4
	CMC	251.5 ± 9.6	6.98 ± 0.22	238.49 ± 9.6	34.16