Fig. 2.

Western blot analysis for ADAMTS-5, −8, −9, and −10 produced by cultured human synovial cells. a: Dose-dependency study. Confluent cells were incubated with or without Hb for 24 h, then the conditioned media was collected. M: marker, C: control (without Hb); 25, 50, and 100 μg/ml of Hb were administered. The collected media was analyzed by Western blotting using antibodies against ADAMTS-5, −8, −9, and −10. b: Time-course study. Confluent cells were incubated with 100 μg/ml Hb, the conditioned media were collected at 6, 12, and 24 h