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. 2017 Nov 14;18:449. doi: 10.1186/s12891-017-1815-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — ELISA analysis for time-course study of ADAMTS-5 and -9 produced by cultured human synovial cells. a: ELISA analysis for the time-course response of ADAMTS-5 to Hb. Confluent cells were incubated with or without Hb (100 μg/ml), then the conditioned media were collected at 6, 12, and 24 h. The level of ADAMTS-5 showed time-course dependency, and there was significantly higher expression at 6, 12, and 24 h after Hb stimulation (p < 0.05). b: ELISA analysis for the time-course response of ADAMTS-9 to Hb. ADAMTS-9 was highly expressed at 6, 12, and 24 h significantly after Hb stimulation compared with the control. (p < 0.05)