Table 2.
Ethanol productivity and ethanol yield of STB5 and YAP1 deletion mutants and transformants in flask cultivations
| Strains, mutants and transformantsa | Inhibitor | Q 16h (g/l/h)b | q 16h (g/g/h)b | Y E/G (g/g)c |
|---|---|---|---|---|
| BY4741 | N/Ad | 0.58 ± 0.01 | 3.93 ± 0.04 | 0.463 ± 0.011 |
| Coniferyl aldehyde | NDTD | NDTD | 0.349 ± 0.014 | |
| HMF | 0.08 ± 0.03 | 0.58 ± 0.15 | 0.222 ± 0.007 | |
| M-Y | N/A | 0.55 ± 0.04 | 3.85 ± 0.16 | 0.437 ± 0.031 |
| Coniferyl aldehyde | NDTD | NDTD | NDTD | |
| HMF | 0.12 ± 0.02 | 0.81 ± 0.09 | 0.182 ± 0.007 | |
| M-S | N/A | 0.52 ± 0.03 | 3.62 ± 0.08 | 0.413 ± 0.021 |
| Coniferyl aldehyde | NDTD | NDTD | 0.375 ± 0.023 | |
| HMF | NDTD | NDTD | NDTD | |
| SIY | N/A | 0.54 ± 0.02 | 3.67 ± 0.08 | 0.434 ± 0.020 |
| Coniferyl aldehyde | NDTD | NDTD | NDTD | |
| HMF | 0.11 ± 0.01 | 0.74 ± 0.01 | 0.241 ± 0.005 | |
| YIS | N/A | 0.55 ± 0.01 | 3.91 ± 0.03 | 0.440 ± 0.005 |
| Coniferyl aldehyde | NDTD | NDTD | 0.317 ± 0.005 | |
| HMF | 0.04 ± 0.01 | 0.28 ± 0.01 | 0.115 ± 0.023 |
NDTD none detected
aBY4741, BY4741 host strain; M-Y, deletion mutant of YAP1; M-S, deletion mutant of STB5; SIY, STB5 overexpressed in deletion mutant of YAP1; YIS, YAP1 overexpressed in deletion mutant of STB5
bCalculations based on the results within the first 16 h of fermentation. Q is the volumetric ethanol productivity, and q is the specific ethanol productivity
c Y E/G is the ethanol yield on the initial amount of fermentable sugar (glucose). The calculations are based on the maximum ethanol concentrations obtained within 136 h of fermentation
dN/A not applicable; no inhibitor was added to the culture medium