Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| V. anguillarum strains | ||
| M93Sm | Spontaneous Smr mutant of M93 (serotype O2a) | [47] |
| XM420 | Smr Cmr; icd insertional mutant | This study |
| ES422 | Smr; Restored icd strain | This study |
| XM440 | Smr Cmr; sucA insertional mutant | This study |
| XM450 | Smr Cmr; sucC insertional mutant | This study |
| XM460 | Smr Cmr; sdhC insertional mutant | This study |
| XM470 | Smr Cmr; fumA insertional mutant | This study |
| XM410 | Smr Cmr; mdh insertional mutant | This study |
| XM430 | Smr Cmr; cra insertional mutant | This study |
| E. coli strains | ||
| SM10 | thi thr leu tonA lacY supE recA RP4–2-Tc::Mu::Km (λ pir) | [48] |
| S100 | Kmr; Sm10 containing plasmid pNQ705–1 | [49] |
| Q420 | Kmr Cmr; Sm10 containing plasmid pNQ705-icd | This study |
| Q440 | Kmr Cmr; Sm10 containing plasmid pNQ705-sucA | This study |
| Q450 | Kmr Cmr; Sm10 containing plasmid pNQ705-sucC | This study |
| Q460 | Kmr Cmr; Sm10 containing plasmid pNQ705-sdhC | This study |
| Q470 | Kmr Cmr; Sm10 containing plasmid pNQ705-fumA | This study |
| Q410 | Kmr Cmr; Sm10 containing plasmid pNQ705-mdh | This study |
| Q430 | Kmr Cmr; Sm10 containing plasmid pNQ705-cra | This study |
| Plasmid | ||
| pNQ705–1 | Cmr; suicide vector with R6K origin | [49] |
| pNQ705-icd | Cmr; For icd insertional mutant | This study |
| pNQ705-sucA | Cmr; For sucA insertional mutant | This study |
| pNQ705-sucC | Cmr; For sucC insertional mutant | This study |
| pNQ705-sdhC | Cmr; For sdhC insertional mutant | This study |
| pNQ705-fumA | Cmr; For fumA insertional mutant | This study |
| pNQ705-mdh | Cmr; For mdh insertional mutant | This study |
| pNQ705-cra | Cmr; For cra insertional mutant | This study |