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. 2017 Nov 14;18:32. doi: 10.1186/s12860-017-0148-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of cRGDfC peptide on mESC morphology and viability when cultured in a dish without the presence of collagen matrix. (a) mESC with LIF and (b) mESC without LIF remained attached and showed no visible differences in cell morphology; (c) mESC with LIF + cRGDfC and (d) mESC without LIF + cRGDfC formed round shaped aggregates and detached from the dish. Scale bar, 200 μm. The morphology of aggregates formed in the presence (e) or absence (f) of LIF was also examined using DAPI staining with 3D confocal imaging and reconstruction. Scale bar, 100 μm. (g) Cell viability significantly decreased in the presence of LIF and cRGDfC to 50% in comparison to mESC w/LIF