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Relative mRNA gene expression of pluripotent markers in the presence of cRGDfC, w/ and w/o LIF (a-d). Supplementation of 0.5 mM cRGDfC peptide down-regulated mESC pluripotency in the presence of LIF. Mean ± SD, n = 3 independent experimental replicates. (***;p < 0.0001; **;p < 0.001; *;p < 0.05). Flow cytometry validation of PCR data (e-i). Representative data from 3 independent experimental replicates
