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. 2017 Nov 14;15:82. doi: 10.1186/s12951-017-0317-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Observation of 3T6 cells transfected with plasmid EGFP (pEGFP). a Untreated 3T6 cells; b Ed-PYP treated 3T6 cells; c free pEGFP; d PEI–pEGFP; e lipofectamine 2000-pEGFP; f–h Ed-PYP–pEGFP nanoparticles prepared at 20:1, 40:1, and 80:1, respectively