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. 2017 Nov 14;15:82. doi: 10.1186/s12951-017-0317-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Protein-expression measurement. a Western blotting indicated the expression of the Ascl1, Brn4, and Tcf3 protein, using β-actin protein as the control. Lane 1, untreated 3T6 cells; lane 2, Ed-PYP treated 3T6 cells; lane 3, free plasmid group Ascl1, Brn4, Tcf3; lane 4, PEI-pABT; lane 5, Lipofectamine 2000 (Lip2000)-pABT; lane 6–8, 3T6 cells transfected with Ed-PYP–pABT nanoparticles prepared at 20:1, 40:1 and 80:1, respectively. b A quantitative analysis of the relative expression levels of Ascl1, Brn4, and Tcf3 (mean ± standard deviation of measurements from three replicates). *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t-test