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. 2017 Nov 14;17:103. doi: 10.1186/s12935-017-0473-z

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — CBFA2T2 is required for cancer stem cell state of RCC cells. a 786-O and A-498 cells were treated with siRNA against CBFA2T2 or control siRNA for 24 h and subjected to the Aldefluor assay. Proportions of the ALDH-positive cell subpopulation in total cancer cells were indicated in the figure. b, c 786-O and A-498 cells were plated into ultra low-attachment 24-well plates at a density of 1000/well and cultured in tumor sphere medium containing siRNA against CBFA2T2 or control siRNA. Tumor spheres were photographed (b) and tumor sphere numbers were counted (c). The data are presented as the mean ± standard deviation with three independent experiments. **indicates p < 0.01