Fig. 4.

The thrombolysis activity had measured with the fibrin plate and TEG analyzer. a Thrombolytic activity of Sak determined by fibrin plate. Stoma 1: 0.1 mg PEG-Sak-C79G; Stoma 2: 0.1 mg PEG-Sak-C82L; Stoma 3: 0.1 mg PEG-Sak-C84K; Stoma 4: 0.1 mg PEG-Sak-C97A; Stoma 5: 0.1 mg PEG-Sak-C104R; Stoma 6: 0.1 mg BSA; Stoma 7: 5.0 × 10³ IU unmodified Sak; Stoma 8: 5.0 × 10³ IU urokinase; Stoma 9: 5.0 × 10³ IU tPA. b Diameters of the lysis dots on the fibrin plate from at four independent evaluations. (Values are the mean ± SD, n = 3; *P < 0.05 compared with wild-type Sak. #P < 0.05 compared with urokinase and tPA). c Thrombolysis activity of Sak determined by TEG analyzer. Ly30, the percent lysis 30 min after maximum amplitude. (Values are the mean ± SD, n = 10; *P < 0.05 compared with wild-type Sak). d Test results of the wild-type and five PEGylated SAKs by TEG analyzer