Fig. 3.

Fine-mapping of the Crd locus in horse and whole-genome sequencing identify a candidate variant in the coil1A domain of KRT25 that affects an amino-acid residue conserved among placental mammals. a The IBD segment delineated by haplotype analysis comprises 36 genes, including 10 genes encoding keratin proteins (KRT-10, -12, -20, -23, -24, -25, -26, -27, -28, -222). b Multispecies alignment of KRT25 orthologues shows a perfect conservation of amino acid R89. The p.R89H variant affects the coil1A domain within the α-helical rod domain of KRT25 protein. Ensembl accession numbers for the wild type transcript of each species are ENSTBEP00000001955, ENSETEP00000006824, ENSEEUP00000013767, ENSMUSP00000048439, ENSCAFP00000032235, ENSSSCP00000018507, ENSP00000310573, ENSBTAP00000040707, ENSECAP00000011587, ENSPVAP00000010713, ENSLAFP00000005738 and ENSOCUP00000007766 in order of appearance