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. 2017 Nov 7;28(23):3156–3164. doi: 10.1091/mbc.E16-07-0520

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© 2017 Broussard, Yang, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Modulating the DSM–IF connection leads to alterations in cell traction and tugging forces. (A) Western blot showing the expression of endogenous DP and doxycycline-induced expression of GFP-tagged DP variants. GAPDH is shown as a loading control. (B) Superresolution micrographs of control cells (not induced) and cells induced to express GFP-tagged DP variants are shown. The GFP-tagged DP variants are shown in green, keratin IFs are shown in white, and DAPI indicates nuclei in blue. (C) The average traction force per pillar and the average intercellular force are shown for control cells and cells induced to express the indicated DP variants. Error bars represent the standard error of the mean from at least 18 cells from three independent experiments. *, p < 0.05; N.S., not significant. (D) Representative Western blot indicating knockdown of endogenous DP in cells treated with either nontargeting siRNA (siCtl) or siRNA targeting DP (siDP). Tubulin is shown as a loading control. (E) The average traction force per pillar and the average intercellular force are shown for cells treated with either nontargeting siRNA (siCtl) or siRNA targeting DP (siDP). Error bars represent the standard error of the mean from at least 30 cells from three independent experiments. *, p < 0.0001.