TABLE 1:
Breast cancer cells analyzed without drug and after trastuzumab exposure.
| Phenotype | Number of cells | Number of labels | Label surface density/μm2 | Analyzed area/μm2 |
|---|---|---|---|---|
| Ruffled bulk cells | 11 | 86,003 | 93 ± 58 | 792 |
| Flat cells | 3 | 9084 | 61 ± 27 | 139 |
| Stem-like cells | 12 | 27,785 | 47 ± 23 | 779 |
| HCC1954 | 7 | 44,879 | 84 ± 33 | 534 |
| After 3-min exposure to trastuzumab: | ||||
| Ruffled bulk cells | 19 | 112,014 | 72 ± 38 | 1713 |
| Flat cells | 5 | 21,000 | 45 ± 23 | 410 |
| Stem-like cells | 7 | 34,825 | 52 ± 23 | 721 |
| HCC1954 | 7 | 28,238 | 53 ± 27 | 530 |
SKBR3 cells were divided into three phenotype groups. One group was found for HCC1954 cells. QD labels attached to membrane-bound HER2 receptors were imaged with STEM. The cumulative number of labels reflects the amount of data used for calculating g(r) of a group. The label surface density including SD represents the average value of the surface densities of individual cells.