FIGURE 6:

Static cross-linkers increase likelihood of stalled “tug-of-war” between opposing motors. (A) Sample MT trajectories in the absence of static cross-linkers (x_on = 0), showing position as a function of time. Parameters: d_on = 0.1 s⁻¹, k_on = 0.01 s⁻¹, Table 1. Legend applies to A and B. (B) Sample MT trajectories in the presence of static cross-linkers (x_on = 0.003 s⁻¹). (C) Bivariate histogram of the attachment numbers of cytoplasmic dynein and kinesin-1 for a 1 μm MT in the absence of cross-linkers (corresponding to the dashed blue line in A). (D) Bivariate histogram of the attachment numbers of cytoplasmic dynein and kinesin-1 for a 10-μm MT in the absence of cross-linkers (corresponding to the solid red line in A). (E) Top: Histogram of dynein and kinesin attachment numbers for a 1-μm MT in the presence of cross-linkers (corresponding to the dashed blue line in B). Bottom: Histogram of dynein and cross-linker attachment numbers for the same trajectory. (F) Histogram of dynein and kinesin attachment (top) and corresponding histogram of dynein and cross-linker attachment (bottom) for a 10-μm MT in the presence of cross-linkers (corresponding to the solid red line in B)