FIGURE 6:

The essential region of the C-terminal domain of Spc110. Row 1 diagrams the arrangement of the C-terminal region. Cyan and red depict locations of alternating heptad repeats with >90% probability of forming a dimeric coiled-coil, whereas purple indicates a drop in probability to 60% (Delorenzi and Speed, 2002; Alva et al., 2016). Viability was assessed as described in the Supplemental Methods. Information from the literature is indicated: 1. Sundberg et al., 1996; 2. Kilmartin and Goh, 1996; 3. Stirling et al., 1996; 4. Geiser et al., 1993; 5. Kilmartin et al., 1993; 6. Sundberg and Davis, 1997.