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. 2017 Nov 7;28(23):3349–3359. doi: 10.1091/mbc.E17-06-0359

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© 2017 Imai et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Difference in DNA density between pericentric heterochromatin foci and euchromatin. (A) Typical confocal images of Hoechst 33342 and H3.1-EGFP signals in live NIH3T3 cells. Large foci in the nuclei are pericentric heterochromatin foci. Scale bar: 5 µm. (B, C) Differences in signal intensity between the heterochromatin (Hch) and surrounding euchromatin (Ech) regions in live NIH3T3 cells. Signal intensity ratios: 7.5 (Hoechst 33342, B) and 5.5 (H3.1-EGFP, C). n = 18 for Hoechst 33342 staining and n = 16 for H3.1-EGFP. (D) Estimated composition of the pericentric foci and euchromatin in live cells. Note that nonnucleosomal materials (nonhistone proteins, RNAs) were dominant in both chromatin regions. For details, see Materials and Methods.