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. 2017 Nov 7;28(23):3349–3359. doi: 10.1091/mbc.E17-06-0359

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© 2017 Imai et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Density of condensed inactive human X chromosome. (A) OPD maps (first row) and DNA staining (second row) of live NIH3T3 cells (left) and RPE1 cells (right). Note that the RPE1 cell has a brightly stained area attached to the nuclear envelope (arrowhead), which is likely to be an inactive X chromosome. High-intensity regions in the nuclei of OPD maps are nucleoli (also see Supplemental Figure S4). (B) Fluorescence images of DNA staining (DAPI, upper) and α-H3K9me3-immunostaining of FA-fixed RPE1 cells. Regions surrounding inactive X chromosomes seem to be free of H3K9me3 marks. (C) The density of the nucleoplasm (euchromatin, Ech) and inactive X chromosome (heterochromatin, Hch) in RPE1 cells (density ratio: 1.48, n = 10 for each).