FIGURE 4:

γ-Interferon treatment differentially affects PD1 transcription in core promoter mutants. (A) Normalized histograms of PD1 RNA expression in B-lymphocytes from WT and core promoter mutant lines stimulated in the absence or presence of γ-interferon. Gray, no γ-interferon; red, + γ-interferon. (B) Expression of core promoter mutants relative to WT in the presence or absence of γ-interferon, as determined from mean expression levels in A. (C) Differential bursting kinetics upon γ-interferon treatment in WT and mutant PD1 genes. Triangles indicate γ-interferon–treated samples in respective panels.