Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 7;28(23):3371–3382. doi: 10.1091/mbc.E17-03-0136

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Reinhard et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 1: — S1P promotes endothelial barrier function: analysis toward a single cell level. (A) ECs were grown to a semiconfluent monolayer, stimulated with S1P (500 nM), and stained for F-actin, phospho-paxillin, and VE-cadherin. Arrowheads highlight protruding areas. Scale bar = 25 μm. (B) ECs were grown to a monolayer on ECIS electrodes and stimulated with S1P at t = 0.3 h. Endothelial resistance (4000 Hz) was measured using the ECIS, normalized to the value at t = 0, and plotted versus time. (C) ECs, transiently transfected with mTq2, were grown to a monolayer. Local changes in the area of a single cell were measured before and after stimulation with S1P at t = 0:30 min. Graph displays a representative dataset of n = 9. (D) Corresponding image of the plot in C. Colors in the right image represent area change after S1P stimulation (look-up table panel on the right). Scale bar = 25 μm.