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. 2017 Nov 7;28(23):3371–3382. doi: 10.1091/mbc.E17-03-0136

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© 2017 Reinhard et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — SEW2871 promotes endothelial barrier function and activates Gα_i, Rac1, and Cdc42. (A) ECs were grown to a monolayer on ECIS electrodes and stimulated with SEW2871 (5 μM) at t = 0.3 h after start of the analysis. Endothelial resistance (4000 Hz) was measured using the ECIS. (B) Normalized mean YFP/CFP ratio traces (±SEM) of Gα_i FRET sensor-expressing ECs (n = 14) stimulated with SEW2871 (5 μM) at t = 55 s. (C) Normalized mean YFP/CFP ratio traces (±SEM) of Gα₁₃ FRET sensor-expressing ECs (n = 16) stimulated with SEW2871 (5 μM) at t = 55 s. (D) Normalized mean YFP/CFP ratio traces (±SEM) for Rac1 (n = 16), Cdc42 (n = 8), and RhoA (n = 6) FRET sensor-expressing ECs before and after stimulation with SEW2871 (5 μM) at t = 1 min, 50 s. (E) Zoom of normalized mean YFP/CFP ratio traces (±SEM) corresponding to the first 7 min of D. Dashed lines highlight maximal YFP/CFP ratio changes of Rac1 and Cdc42.