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. 2017 Nov 7;28(23):3371–3382. doi: 10.1091/mbc.E17-03-0136

FIGURE 6:

FIGURE 6:

i signaling is required for S1P-mediated Rac1/Cdc42 activation and endothelial barrier promotion. (A–C) Normalized mean YFP/CFP ratio traces (±SEM) for Rac1 (n = 12), Cdc42 (n = 9), and RhoA (n = 13) FRET sensor-expressing ECs (pretreated with 100 ng/ml PTX) before and after stimulation with S1P (500 nM) at t = 1:50. (D) ECs were grown to a monolayer on ECIS electrodes, pretreated with water or PTX dissolved in water (100 ng/ml), and stimulated with S1P (500 nM) at t = 50 min. Endothelial resistance (4000 Hz) was measured using the ECIS. (E) EC were grown to a semiconfluent monolayer, pretreated with PTX as above, stimulated with S1P (500 nM), and stained for F-actin, phospho-paxillin, and VE-cadherin. Scale bar = 25 μm.